Regulatory

Part:BBa_K2201008:Design

Designed by: Christopher M. Whitford   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


pSC101 temperature sensitive origin of replication


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 199
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 199
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 199
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence simillar to pMAK


Source

pSC101

References